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Cytogenetic instability and the accumulation of heavy metals in the tissues of pelophylax ridibundus living in the urban area

Published in «Water: chemistry and ecology» № / 2019 , p. 93-98.
Heading:

 

Romanova E.B.
Ryabinina E.S.
Shapovalova K.V.
Boryakov A.V.

Summary:
Analysis of the intensity of mutagenesis and cytogenetic resistance of the organism is an important approach to the study of adaptive strategies of species. The aim of the work was to assess the percentage of erythrocyte with micronuclei in the peripheral blood and the accumulation of heavy metals (Mn, Cu, Cr, Al, Zn, Sr) in the organs of Pelophylax ridibundus in the urban environment and natural transformation. Hydrochemical analysis determined the content of a number of pollutants in the studied reservoirs: iron, manganese, copper, chromium, nitrate and nitrite ions, lead, cobalt, nickel, zinc, sulfates and sulfides and calculated the specific combinatorial index of water pollution (SCIWP). The number of erythrocyte, leukocyte and leukogram were determined by the conventional method. Four types of micronuclei were isolated in blood erythrocytes and their areas were calculated (μm2). The content of heavy metals in the tissues of Pelophylax ridibundus was determined by the atomic-emission method and the coefficients of biological absorption and accumulation were calculated. In conditions of increased pollution of the aquatic environment in the blood of lake frogs leukopenia was detected, indicating a violation of normal blood formation and a decrease in the body's immune defense. Against the background of a low leukocyte count, there was a decrease in the number of lymphocytes and an increase in the proportion of neutrophilic granulocytes. All populations were characterized by a high content of erythrocytes with micronuclei, the most frequently attached and absent rod-shaped micronuclei. The average area of the attached micronuclei was (1.2 ± 0.04) μm2. In high concentrations, heavy metals are found in the spleen (chromium) and bones (zinc, strontium) of frogs. Copper ions accumulated in the heart muscle and liver. The maximum biological absorption coefficient is established for Mn, Cu, Cr, Al, and Zn. The results obtained indicated a high degree of accumulation pollutants of natural and technogenic origin, an increase in cytogenetic instability and a decrease in the protective immune responses of Pelophylax ridibundus living in urban environments.

Keywords: bioindication, heavy metals, micronucleus test, Pelophylax lessonae

Bibliographic link:
Romanova E.B., Ryabinina E.S. , Shapovalova K.V., Boryakov A.V. Cytogenetic instability and the accumulation of heavy metals in the tissues of pelophylax ridibundus living in the urban area // Water: chemistry and ecology. — 2019. — № . — c. 93-98. — http://watchemec.ru/en/article/29790/

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